Recombinant Milk Fat Globule EGF Factor 8 (MFGE8)

Residues: Cys73~Arg421

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: P70490

Host: E. coli

Subcellular Location: Membrane, Peripheral

membrane protein, Secreted.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 69.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month.Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

CFHDAKCL VTEDTQRGDI FTEYICQCPV GYSGIHCELG CSTKLGLEGG AIADSQISAS

S V Y M G F M G L Q RW G P E L A R LY RT G I V N AW TA S S Y D S K P W I Q V D F L R K M R V S

GVMTQGASRA GRAEYLKTFK VAYSLDGRRF EFIQDESGTG DKEFMGNQDN NSLKINMFNP

TLEAQYIRLY PVSCHRGCTL RFELLGCELH GCSEPLGLKN NTIPDSQITA SSSYKTWNLR

AFGWYPHLGR LDNQGKINAW TAQSNSAKEW LQVDLGTQKK VTGIITQGAR DFGHIQYVAS

YKVAHSDDGV QWTVYEEQGT SKVFQGNLDN NSHKKNIFEK PFMARYVRVL PLSWHNRITL

R