Recombinant Human Bone Morphogenetic Protein 2/BMP-2

Instruction Manual!

Product name:	Recombinant Human Bone Morphogenetic Protein 2/BMP-2
Source:	E.coli
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Lyophilized from a 0.2 μm filtered solution of 50mM HAc .
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	E.coli
Description	Recombinant Human Bone Morphogenetic Protein 2 is produced by our E.coli expression system and the target gene encoding Gln283-Arg396 is expressed.
Names	Bone Morphogenetic Protein 2, BMP-2, Bone Morphogenetic Protein 2A, BMP-2A, BMP2, BMP2A
Accession #	P12643
Formulation	Lyophilized from a 0.2 μm filtered solution of 50mM HAc .
Shipping	The product is shipped at ambient temperature.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 50mM Acetic acid. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Biological Activity	ED50 is less than 50 ng/ml. Specific Activity of 2.0 x 10^4 IU/mg
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQ TLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
Background	Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
References	Wu T,et al.miR-30 Family Members Negatively Regulate Osteoblast Differentiation PMID:22253433 http://www.ncbi.nlm.nih.gov/pubmed/22253433