Recombinant Regucalcin (RGN)

Instruction Manual!

Product name:	Recombinant Regucalcin (RGN)
Source:	E. coli
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE; WB; ELISA; I
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month.Aliquot and store at -80℃ for 12 months
UOM:	100ug/50ug/200ug/1mg/1g

[ PROPERTIES ]

Residues: Gln65~Gly299

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q15493

Also known as: RC; SMP30; GNL; Senescence Marker Protein-30; Gluconolactonase

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 29.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8^oC for one month.

Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

QSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN RFNDGKVDPA GRYFAGTMAE

ETAPAVLERH QGALYSLFPD HHVKKYFDQV DISNGLDWSL DHKIFYYIDS LSYSVDAFDY

DLQTGQISNR RSVYKLEKEE QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL

PVDKTTSCCF GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG