Alpha-2-Macroglobulin (a2M)

ICB017Ra01

Instruction manual

 FOR IN VITRO USE AND RESEARCH USE ONLY

 NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  5th Edition (Revised in January, 2013)

[ DESCRIPTION ]                                                                       
                                                                                                                                  Synonyms: a2M, A2m1                                                             

Species: Rat                                                                              

 Size: 100µg                                                                               

 Source: Escherichia coli-derived                                               

 Subcellular Location: Secreted.                                              

 [ PROPERTIES ]                                                                        

 Residues: Arg216~Phe437 (Accession # P06238), with N-terminal His-Tag.

 Grade & Purity: >95%, 28kDa as determined by SDS-PAGE reducing conditions.

 Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.

 Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

 Applications: SDS-PAGE; WB; ELISA; IP.

 (May be suitable for use in other assays to be determined by the end user.)

 Predicted Molecular Mass: 26.1kDa

 Predicted isoelectric point: 6.4

 [ PREPARATION ]

 Reconstitute in sterile PBS, pH7.2-pH7.4.

 [ STORAGE AND STABILITY ]

 Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8^oC for one month.

 Aliquot and store at -80^oC for 12 months.

 Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

 [ SEQUENCES ]

 The target protein is fused with N-terminal His-Tag, its sequence is listed below.

 MGHHHHHHSGS-   RTVEH    PFSVEEFVLP    KFEVRVTVPE    TITILEEEMN    VSVCGIYTYG

 KPVPGRVTVN ICRKYSNPSN CFGEESVAFC EKLSQQLDGR GCFSQLVKTK SFQLKRQEYE

 MQLDVHAKIQ EEGTGVEETG KGLTKITRTI TKLSFVNVDS HFRQGIPFVG QVLLVDGRGT

 PIPYETIFIG ADEANLYINT TTDKHGLARF SINTDDIMGT SLTVRAKYKD SNACYGF