Alpha-2-Macroglobulin (a2M)

ICB017Hu01

Alpha-2-Macroglobulin (a2M)

Organism: Homo sapiens (Human)
Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ DESCRIPTION ]

Protein Names: Alpha-2-Macroglobulin                                  

Synonyms: a2M, CPAMD5                                                                                                                          Species: Human                                                                     

Size: 100µg                                                                            

Source: Escherichia coli-derived                                            

Subcellular Location: Secreted.                                           

[ PROPERTIES ]                                                                     

Residues: Ser616~Val856 (Accession # P01023), with N-terminal His-Tag.
Grade & Purity: >95%, 30kDa as determined by SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 28.4kDa

Predicted isoelectric point: 5.7

 [ PREPARATION ]

 Reconstitute in sterile PBS, pH7.2-pH7.4.

 [ STORAGE AND STABILITY ]

 Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8^oC for one month.
Aliquot and store at -80^oC for 12 months.

 Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

 [ SEQUENCES ]

 The target protein is fused with N-terminal His-Tag, its sequence is listed below.

 MGHHHHHHSGSEF- SSVYN LLPEKDLTGF PGPLNDQDNE DCINRHNVYI NGITYTPVSS

 TNEKDMYSFL        EDMGLKAFTN        SKIRKPKMCP         QLQQYEMHGP         EGLRVGFYES

 DVMGRGHARL VHVEEPHTET VRKYFPETWI WDLVVVNSAG VAEVGVTVPD TITEWKAGAF

 CLSEDAGLGI  SSTASLRAFQ  PFFVELTMPY  SVIRGEAFTL  KATVLNYLPK  CIRVSVQLEA

 SPAFLAVPVE KEQAPHCICA NGRQTV