Recombinant Human Platelet-derived Growth Factor Receptor Alpha/PDGF Rα

Instruction Manual!

Product name:	Recombinant Human Platelet-derived Growth Factor Receptor Alpha/PDGF Rα
Source:	Human Cells
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	Human Cells
Description	Recombinant Human Platelet-derived Growth Factor Receptor Alpha is produced by our Mammalian expression system and the target gene encoding Gln24-Glu524 is expressed with a 6His at the C-terminus.
Names	Platelet-derived growth factor receptor alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor;CD140 antigen-like family member A; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD140a
Accession #	P16234
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Shipping	The product is shipped at ambient temperature.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	QLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVS SASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTT DPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEM EALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVK DSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRIS WLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQV PSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHS RDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSEHHHHHH
Background	Platelet-derived Growth Factor Receptor Alpha (PDGF Rα) is an enzyme that belongs to the class III subfamily of receptor tyrosine kinases.It is a type I transmembrane glycoprotein, and can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, PDGF Rα is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture, but is lowly expressed in most mesenchymal cells. PDGF Rα autophosphorylates upon dimerization, activating signaling cascades in PI-3kinase Ras-MAP kinase, and PLC-γ pathways. PDGF Rα has infulence on local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial ,cardiac neural crest and interstitial kidney mesenchyme.