Recombinant Human Phosphatidylinositol 4-Kinase β/PI4KB

Instruction Manual!

Product name:	Recombinant Human Phosphatidylinositol 4-Kinase β/PI4KB
Source:	Human Cells
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Supplied as a 0.2 μm filtered solution of 25mM Tris.HCl,pH7.3,100mM glycine,10%glycerol.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	Human Cells
Description	Recombinant Human PI4KB is produced by our Mammalian expression system and the target gene encoding Met1-Met801 is expressed with a 6His tag at the C-terminus.
Names	Phosphatidylinositol 4-Kinase Beta, PI4K-Beta, PI4KBeta, PtdIns 4-Kinase Beta, NPIK, PI4K92, PI4KB, PIK4CB
Accession #	Q9UBF8-2
Formulation	Supplied as a 0.2 μm filtered solution of 25mM Tris.HCl,pH7.3,100mM glycine,10%glycerol.
Shipping	The product is shipped on dry ice/ice packs.
Storage	Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MGDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGG VAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQSWL LRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIK PYIVHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPA PDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEPVRLAPEREFIKSLMAIGKRLA TLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLE CENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVE LPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKE PWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKI LVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCY LLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYK MLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDG SMRSITTKLYDGFQYLTNGIMVDHHHHHH
Background	Phosphatidylinositol 4-Kinase β (PI4KB) belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. PI4KB contains one PI3K/PI4K domain and one PIK helical domain. It is highly expressed in heart, skeletal muscle, pancreas, testis and ovary,; it is lowly expressed in liver tissue. PI4KB kinase activity can be inhibited by wortmannin, and increased by interacting with NCS1/FREQ. PI4KB phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). In addition, PI4KB may regulate Golgi disintegration/reorganization during mitosis.