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Recombinant Epidermal Growth Factor Receptor (EGFR)
Instruction Manual!
| Product name: | Recombinant Epidermal Growth Factor Receptor (EGFR) |
| Source: | E. coli |
| Purity: | ＞95% |
| Buffer Formulation: | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcos |
| Applications: | SDS-PAGE; WB; ELISA; IP |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month.Aliquot and store at -80℃ for 12 months |
| UOM: | 100ug/50ug/200ug/1mg/1g |
[ PROPERTIES ]
Residues: Lys713~Asp984 (Accession # P00533), with two N-terminal Tags, His-tag and S-tag.
Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type I membrane protein. Endoplasmic reticulum membrane; Golgi apparatus membrane; Endosome. Endosome membrane. Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 37.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MH H H H H H S S G LV P R G S G M K E TA A A K F E R Q H MD S P D L G T D D D D K A M A D I G S
EFELRRQAC- KKIKVLGS GAFGTVYKGL WIPEGEKVKI PVAIKELREA TSPKANKEIL
DEAYVMASVD NPHVCRLLGI CLTSTVQLIT QLMPFGCLLD YVREHKDNIG SQYLLNWCVQ
IAKGMNYLED RRLVHRDLAA RNVLVKTPQH VKITDFGLAK LLGAEEKEYH AEGGKVPIKW
MALESILHRI YTHQSDVWSY GVTVWELMTF GSKPYDGIPA SEISSILEKG ERLPQPPICT
IDVYMIMVKC WMIDADSRPK FRELIIEFSK MARDPQRYLV IQGD
