Recombinant Human Serpin B5/SERPINB5/Maspin

Instruction Manual!

Product name:	Recombinant Human Serpin B5/SERPINB5/Maspin
Source:	E. coli
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	E. coli
Description	Recombinant Human Serpin B5/Maspin is produced by our E.coli expression system and the target gene encoding Met1-Ser231 is expressed.
Names	Serpin B5, Maspin, Peptidase Inhibitor 5, PI-5, SERPINB5, PI5
Accession #	P36952-2
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping	The product is shipped at ambient temperature.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKD VPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKG QINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAAC SSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS
Background	Serpin B5 is a secretive protein that belongs to the Serpin (Serine Protease Inhibitor) family and Ov-serpin subfamily. Serpin B5 is expressed in the prostate, testis, intestine, tongue, lung, and thymus. Serpin B5 exhibits no serine protease inhibitory activity. Serpin B5 also functions as tumor suppressor an angiogenesis inhibitor. It has been shown that Serpin B5 blocks the growth, invasion, and metastatic properties of mammary tumors. Furthermore, high expression of Serpin B5 is linked to squamous cell carcinoma in non-small-cell lung cancer.