Recombinant Human Baculoviral IAP Repeat-Containing Protein 5/BIRC5/Survivin

Instruction Manual!

Product name:	Recombinant Human Baculoviral IAP Repeat-Containing Protein 5/BIRC5/Survivin
Source:	E. coli
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 0.1M NaCl, pH 7.5.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	E. coli
Description	Recombinant Human BIRC5 is produced by our E.coli expression system and the target gene encoding Met1-Asp142 is expressed.
Names	Baculoviral IAP Repeat-Containing Protein 5, Apoptosis Inhibitor 4, Apoptosis Inhibitor Survivin, BIRC5, API4, IAP4
Accession #	O15392
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 0.1M NaCl, pH 7.5.
Shipping	The product is shipped at ambient temperature.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELE GWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKK VRRAIEQLAAMD
Background	Baculoviral IAP Repeat-Containing Protein 5 (BIRC5) belongs to the IAP family. BIRC5 exists as a homodimer in the apo state and as a monomer in the CPC-bound state. BIRC5 contains one BIR repeat and is expressed only in fetal kidney and liver, and to lesser extent, lung and brain. BIRC5 functions as a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. BIRC5 is also a component of a chromosome passage protein complex (CPC), which is essential for chromosome alignment and segregation during mitosis and cytokinesis. BIRC5 acts as an important regulator of the localization of this complex.