| ELISA kit |
| Clinical diagnosis |
| Trfia |
| ANTIBODIES |
| CLIA kits |
| Proteins |
| New Products |
| > Human |
| > Canine |
| > Bovine |
| > Equine |
| > Fish |
| > Mouse |
| > Rat |
| > Chicken |
| > Porcine |
| > Rabbit |
| > Goat |
| > Simian |
| > General |
| > Cavia |
| > Caprine |
| > Sheep |
| > Cattle |
Recombinant Matrix Metalloproteinase 2 (MMP2)
Instruction Manual!
| Product name: | Recombinant Matrix Metalloproteinase 2 (MMP2) |
| Source: | E. coli |
| Purity: | >95% |
| Buffer Formulation: | Reconstitute in sterile PBS, pH7.2-pH7.4. |
| Applications: | SDS-PAGE; WB; ELISA; IP. |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month.Aliquot and store at -80℃ for 12 months |
| UOM: | 100ug/50ug/200ug/1mg/1g |
[ PROPERTIES ]
Residues: Tyr445~Cys660 (Accession # P08253), with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 25.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-YGASPD IDLGTGPTPT LGPVTPEICK QDIVFDGIAQ IRGEIFFFKD
RFIWRTVTPR DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER
GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP GFPKLIADAW
NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF GSIKSDWLGC
[ REFERENCES ]
1. Brooks P.C., et al. (1996) Cell 85:683-693.
2. Butler G.S., et al. (1997) Eur. J. Biochem. 244:653-657.
3. Bello L., et al. (2001) Cancer Res. 61:8730-8736.
4. Chattopadhyay N., et al. (2001) J. Cancer Res. Clin. Oncol. 127:653-658.
