Recombinant Mouse FcRn & B2M Heterodimer

Instruction Manual!

Product name:	Recombinant Mouse FcRn & B2M Heterodimer
Source:	Human Cells
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	Human Cells
Description	Recombinant Mouse IgG Fc fragment receptor transporter is produced by our Mammalian expression system and the target gene encoding Ser22-Val301&Ile21-Met119 is expressed with a 6His tag at the C-terminus.
Names	IgG receptor FcRn,Neonatal Fc receptor,FCRN
Accession #	Q61559&P01887
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Shipping	The product is shipped at ambient temperature.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	SETRPPLMYHLTAVSNPSTGLPSFWATGWLGPQQYLTYNSLRQEADPCGAWMWENQVSWYWEKET TDLKSKEQLFLEALKTLEKILNGTYTLQGLLGCELASDNSSVPTAVFALNGEEFMKFNPRIGNWT GEWPETEIVANLWMKQPDAARKESEFLLNSCPERLLGHLERGRRNLEWKEPPSMRLKARPGNSGS SVLTCAAFSFYPPELKFRFLRNGLASGSGNCSTGPNGDGSFHAWSLLEVKRGDEHHYQCQVEHEG LAQPLTVDLDSSARSSVPVVVDHHHHHH&IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIE IQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM
Background	FcRn complex consist of two subunits: IgG receptor FcRn large subunit p51（alpha chain) and Beta-2-microglobulin（Beta chain). The complexes is similar in structure to MHC class I-like heterodimer. Beta-2-microglobulin involved in the presentation of peptide antigens to the immune system. FcRn binds to the Fc region of monomeric immunoglobulins gamma, mediates the uptake of IgG from milk,Possible role in transfer of immunoglobulin G from mother to fetus. A principal component of antibody transport is the neonatal receptor for the Fc portion of immunoglobulin, a heterodimer of a MHC-1 alpha-chain homolog ( FcRn ) and beta-2-microglobulin ( B2M ).