Recombinant Mouse Testican-3/SPOCK3

Instruction Manual!

Product name:	Recombinant Mouse Testican-3/SPOCK3
Source:	Human Cells
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	Human Cells
Description	Recombinant Mouse Testican-3 is produced by our Mammalian expression system and the target gene encoding Ala23-Ile436 is expressed with a 6His tag at the C-terminus.
Names	Testican-3,Spock3
Accession #	Q8BKV0-1
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping	The product is shipped at ambient temperature.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	AAAVAVAGGRSDGGNFLDEKQWLTTISQYDKEVGQWNKFRDEVEDDYFRTWNPGKPFDQALDPAK DPCLKTKCSRHKVCITQDAQTALCISHRRLTHSMKEVGGSHKQWRGLPSSTCKPCPIAYASPVCG SDGHSYSSQCKLEYQACVLGKQISIKCEGRCPCPSDKSMNIGRNVKRACSDLEFREVANRLRDWF KALHESGSQNKKTKALLRPERSRFDTSILPICKDSLGWMFNRLDTNYDLLLDQSELGSIYLDKNE QCTKAFFNSCDTYKDSLISNNEWCYCFQRQQDPPCHTELSNIQKRQGIKKLLGQYIPLCDEDGYY KPTQCHGSVGQCWCVDRYGNEVVGSRINGVADCAIDFEISGDFASGDFREWTDDEGEEDDIMNDK DDIEDDDEDEGDDDDDGDVHDGYIVDHHHHHH
Background	SPOCK3，also called Testican3,is a secreted protein and make up a family of extracellular heparan/chondroitin sulfate proteoglycans. It contains 1 Kazal-like domain and 1 thyroglobulin type-1 domain and mainly expressed in brain. SPOCK3 contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. In addition to their presence in testis, testicans are enriched in brain and have been shown to regulate neuronal attachment and outgrowth.