Recombinant Human Aldehyde Dehydrogenase 1-A3/ALDH1A3

Instruction Manual!

Product name:	Recombinant Human Aldehyde Dehydrogenase 1-A3/ALDH1A3
Source:	E.coli
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mMNaCl, pH7.5,20% Glycerol.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	E.coli
Description	Recombinant Human Aldehyde dehydrogenase 1-A3 is produced by our E.coli expression system and the target gene encoding Met1-Pro512 is expressed with a 6His tag at the N-terminus.
Names	Aldehyde dehydrogenase family 1 member A3, ALDH1A3, Aldehyde dehydrogenase 6, Retinaldehyde dehydrogenase 3, RALDH-3, ALDH6
Accession #	P47895
Formulation	Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mMNaCl, pH7.5,20% Glycerol.
Shipping	The product is shipped on dry ice/ice packs.
Storage	Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MNHKVHHHHHHMATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNP STREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALET MDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPL LMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQ INKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCC TAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKL ECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVF TKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGD KNP
Background	Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), also known as retinaldehyde dehydrogenase 3 (RALDH3), is a member of the aldehyde dehydrogenase family known to metabolize a wide variety of aldehydes. ALDH1A3 specifically oxidizes retinal to retinoic acid (RA) and is differentially expressed in developing embryonic tissues and adult organs. The RA produced by ALDH1A3 in rodents contributes to the development of skin and hair follicles, brain, tooth buds, lungs, olfactory bulbs, kidneys, eyes, skeletal muscle and seminal vesicles. In recent research, ALDH1A3 could be as a marker of cancer stem cell to predict metastasis or clinical prognosis in many cancers.