Recombinant Human Annexin A5/ANXA5

Instruction Manual!

Product name:	Recombinant Human Annexin A5/ANXA5
Source:	E.coli
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 1mM DTT, 10% Glycerol, pH 8.0.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	E.coli
Description	Recombinant Human Annexin A5 is produced by our E.coli expression system and the target gene encoding Ala2-Asp320 is expressed.
Names	Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental Anticoagulant Protein 4, PP4, Placental Anticoagulant Protein I, PAP-I, Thromboplastin Inhibitor, Vascular Anticoagulant-Alpha, VAC-Alpha, ANXA5, ANX5, ENX2, PP4
Accession #	P08758
Formulation	Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 1mM DTT, 10% Glycerol, pH 8.0.
Shipping	The product is shipped on dry ice/ice packs.
Storage	Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDL LDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYE EEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFI TIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMK GAGTDDHTLIRVMVSRSETDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD
Background	Annexin A5 (ANXA5) is a member of the annexin family of calcium-dependent phospholipid binding proteins. ANXA5 is an anticoagulant protein by acting as an indirect inhibitor of the thromboplastin-specific complex. ANXA5 is also a protein kinase C and phospholipase A2 inhibitor. It participate in inflammation, cellular signal transduction, growth and differentiation. ANXA5 binding to phosphatidylserine and sulfatide to regulates coagulability in the blood stream. It also protects sinsuoidal endothelial cells from ischemia reperfusion damage. ANXA5 is important for normal CFTR chloride channel activity.