Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1/UCH-L1

Instruction Manual!

Product name:	Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1/UCH-L1
Source:	E.coli
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Buffer Formulation:	Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
Applications:	Applications:SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

Source	E.coli
Description	Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 is produced by our E.coli expression system and the target gene encoding Met1-Ala223 is expressed with a 6His tag at the C-terminus.
Names	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1, UCHL1
Accession #	P09936
Formulation	Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
Shipping	The product is shipped on dry ice/ice packs.
Storage	Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Amino Acid Sequence	MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKK QIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRA KCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLK DAAKVCREFTEREQGEVRFSAVALCKAALEHHHHHH
Background	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.