Recombinant Secreted Phosphoprotein 2 (SPP2)

Instruction Manual!

Product name:	Recombinant Secreted Phosphoprotein 2 (SPP2)
Source:	Phe30~Glu211
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE; WB; ELISA; IP
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month.Aliquot and store at -80℃ for 12 months
UOM:	100ug/50ug/200ug/1mg/1g

[ PROPERTIES ]

Residues: Phe30~Glu211

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q13103

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.9

Predicted Molecular Mass: 24.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(My be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8^oC for one month.

Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

F PVYDYDPSSL RDALSASVVK VNSQSLSPYL FRAFRSSLKR VEVLDENNLV MNLEFSIRET

TCRKDSGEDP ATCAFQRDYY VSTAVCRSTV KVSAQQVQGV HARCSWSSST SESYSSEEMI

FGDMLGSHKW RNNYLFGLIS DESISEQFYD RSLGIMRRVL PPGNRRYPNH RHRARINTDF