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Recombinant Forkhead Box Protein O1 (FOXO1)
Instruction Manual!
| Product name: | Recombinant Forkhead Box Protein O1 (FOXO1) |
| Source: | >95% |
| Purity: | E.coli |
| Buffer Formulation: | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. |
| Applications: | SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.(May be suitable for use in other assays to be determined by the end user.) |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months. |
| UOM: | 100ug/50ug/200ug/1mg/1g |
[ PROPERTIES ]
Source: Prokaryotic expression.
Host: E. coli
Residues: Arg222~Pro469
Tags: N-terminal His-Tag
Tissue Specificity: Liver.
Subcellular Location: Cytoplasm. Nucleus.
Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.5
Predicted Molecular Mass: 30.3kDa
Accurate Molecular Mass: 22&25&27&33kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8℃for one month.
Aliquot and store at -80℃ for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
