Recombinant Thyroglobulin (TG)

[ PROPERTIES ]

Residues: Ser2083~Ala2333 (Accession # P01266), with

two N-terminal Tags, His-tag and T7-tag.

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl. Predicted isoelectric point: 6.5

Predicted Molecular Mass: 30.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the

protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius

equation.)  The  loss  of  this  protein  is  less  than  5%  within  the  expiration  date  under

appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its

sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SLTEKVSL DSWQSLALSS

VVVDPSIRHF DVAHVSTAAT SNFSAVRDLC LSECSQHEAC LITTLQTQPG AVRCMFYADT

QSCTHSLQGQ NCRLLLREEA THIYRKPGIS LLSYEASVPS VPISTHGRLL GRSQAIQVGT

SW K Q V D Q F L G VP YA A P P L A E RR F Q A P E P L N WT G S W D A S K P RA S C W Q P G T R

TSTSPGVSED CLYLNVFIPQ NVAPNASVLV FFHNTMDREE SEGWPAIDGS FLAAVGNLIV

VTA