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Recombinant Growth Differentiation Factor 11 (GDF11)
Instruction Manual!
| Product name: | Recombinant Growth Differentiation Factor 11 (GDF11) |
| Source: | E. coli |
| Purity: | >90% |
| Buffer Formulation: | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
| Applications: | SDS-PAGE; WB; ELISA; IP. |
| Storage: | Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months. |
| UOM: | 100ug/50ug/200ug/1mg/1g |
[ PROPERTIES ]
Residues: Cys105~Pro341
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9Z217
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.4
Predicted Molecular Mass: 30.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
CCHFHF SPKVMFTKVL KAQLWVYLRP VPRPATVYLQ ILRLKPLTGE GTAGGGGGGR
RHIRIRSLKI ELHSRSGHWQ SIDFKQVLHS WFRQPQSNWG IEINAFDPSG TDLAVTSLGP
GAEGCHPFME LRVLENTKRS RRNLGLDCDE HSSESRCCRY PLTVDFEASG WDWIIAPKRY
KANYCSGQCE YMFMQKYPHT HLVQQANPRG SAGPCCTPTK MSPINMLYFN DKQQIIYGKI
P
[ REFERENCES ]
1. Nakashima M., et al. (1999) Mech. Dev. 80:185-189.
2. Ge G., et al. (2005) Mol. Cell. Biol. 25:5846-5858.
3. Ge G., et al. (2005) Molecular and Cellular Biology 25 (14): 5846–58.
4. Egerman MA., et al. (2015) Cell Metabolism 22 (1): 164–74.
