Recombinant Growth Differentiation Factor 9 (GDF9)

[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E. coli

Residues: Gly320~Arg454

Tags: N-terminal His-Tag

Tissue Specificity: Ovary.

Subcellular Location: Secreted.

Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.1

Predicted Molecular Mass: 16.8kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month.

Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE