Recombinant Three Prime Repair Exonuclease 1 (TREX1)

Instruction Manual!

Product name:	Recombinant Three Prime Repair Exonuclease 1 (TREX1)
Source:	E. coli
Purity:	>95%
Buffer Formulation:	20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Applications:	Positive Control; Immunogen; SDS-PAGE; WB.
Storage:	Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E. coli

Residues: Arg6~Glu369

Tags: N-terminal His-Tag

Tissue Specificity: Thymus, Spleen, Liver, Brain.

Subcellular Location: Nucleus. Cytoplasm. Endoplasmic Reticulum Membrane;

Peripheral Membrane Protein.

Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 42.2kDa

Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the

protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE