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GST Antibody (110-218)
Instruction Manual!
| Product name: | GST Antibody (110-218) |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB, IP, IF, ELISA |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
GST Antibody (110-218)
Catalog Number：IC173175
Product Profile
| ProductName | GST Antibody (110-218) |
|---|---|
| AntibodyType | Tags Antibodies |
| ModificationNotes | Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
|
recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA:
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| Species Reactivity | |
| Concentration | 200 µg/ml |
| Purification | Affinity purified |
Target Information
| AlternativeNames |
epitope corresponding to amino acids 110-218 mapping at the C-terminus of GST of Schistosoma japonicum origin
|
|---|---|
| TissueSpecificity | epitope corresponding to amino acids 110-218 mapping at the C-terminus of GST of Schistosoma japonicum origin |
Database Links
Application
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Application
Western blot analysis of recombinant GST fusion protein.
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Application
Western blot analysis of recombinant GST under reducing conditions.
| ApplicationNotes |
recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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