p-c-Myc Antibody (Thr 58)

Instruction Manual!

Product name:	p-c-Myc Antibody (Thr 58)
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WB, IP, IF, IHC
Storage:	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:	100ug

Catalog Number：IC197365

ProductName	p-c-Myc Antibody (Thr 58)
AntibodyType	Tags Antibodies
ModificationNotes	c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3. Mouse, rat and human c-Myc are phosphorylated at their N-terminal domains on serine and threonine residues, specifically on Thr 58 in response to mitogens.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIPIFIHC
	See 1 citations for p-c-MycAntibody (Thr 58):
Species Reactivity	HumanMouseRat
Concentration	100 µg/ml
Purification	Affinity purified

AlternativeNames	recommended for detection of Thr 58 phosphorylated c-Myc of mouse rat and human origin by WB IP IF and IHC(P) More
Tissue Specificity	recommended for detection of Thr 58 phosphorylated c-Myc of mouse, rat and human origin by WB, IP, IF and IHC(P)

Entrez Gene	4609

Application

Western blot analysis of c-Myc phosphorylation in non-transfected: (A) and human c-Myc transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of c-Myc phosphorylation in non-transfected: (A) and human c-Myc transfected: (B) 293T whole cell lysates.
Application

Antibodies tested include p-c-Myc (Thr 58): (A, B, C) and c-Myc (N-262): (D, E, F).
Application

Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.

Application Notes	See 1 citations for p-c-MycAntibody (Thr 58):

Form	Liquid
StorageInstructions	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.