c-Myc Antibody (N-262)

Instruction Manual!

Product name:	c-Myc Antibody (N-262)
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WB, IP, IF, ELISA, GS, ChIP
Storage:	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:	100ug

Catalog Number：ic206415

Product Name	c-Myc Antibody (N-262)
Antibody Type	Tags Antibodies
ModificationNotes	c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
TestedApplications	WBIPIFELISAGSChIP
	recommended for detection of c-Myc p67 of mouse, rat, human and monkey origin by WB, IP, IF and ELISA:
Species Reactivity	HumanMouseRat
Concentration	200 µg/ml
Purification	Affinity purified

AlternativeNames	epitope corresponding to amino acids 1-262 of c-Myc of human origin (includes transcriptional activation region but not the bHLH-Zip protein-protein interaction domain)
Tissue Specificity	epitope corresponding to amino acids 1-262 of c-Myc of human origin (includes transcriptional activation region, but not the bHLH-Zip protein-protein interaction domain)

Entrez Gene	4609

Application

Nuclear c-Myc in small intestinal adenoma in APC -/+ (20X microscopic magnification). Dilution: 1:80 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. Kindly provided by Dr. Albert J. Fornace Jr., Georgetown University.
Application

Western blot analysis of c-Myc expression in non-transfected: (A) and human c-Myc transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of c-Myc expression in non-transfected: (A) and mouse c-Myc transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of c-Myc expression in COS (A) and Jurkat (B) whole cell lysates.
Application

Antibodies tested include c-Myc (9E10): (A-C), c-Myc (C-33): (D,E) and c-Myc (N-262): (F-H).
Application

Antibodies tested include c-Myc (N-262): (A,B) and p-c-Myc (Thr 58/Ser 62)-R: (C,D).

ApplicationNotes	recommended for detection of c-Myc p67 of mouse, rat, human and monkey origin by WB, IP, IF and ELISA:

Form	Liquid
StorageInstructions	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.