GFP antibody

Instruction Manual!

Product name:	GFP antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	0.02M Potassium Phosphate, 0.15M Sodium Chloride. 0.01% Sodium Azide was added as a preservative.
Applications:	EMELISAICC/IFIHC-FIHC-PIPWB
Storage:	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:	100ug

Catalog Number：IC254457

Product Name	GFP antibody
Antibody Type	Tags Antibodies
Productdescriptio	Rabbit polyclonal to GFPThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. More
Immunogen	Highly purified recombinant GFP made in Escherichia coli

Gene Synonyms	Green Fluorescent Protein
Target	GFP
TissueSpecificity	Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

Application

Western blot of GFP protein detected with anti-GFP antibody (1:1000 dilution) and detected with IRDye® 800-conjugated goat anti-rabbit IgG (1:10,000 dilution). Wild type GFP (0.1 µg) was used to spike 30 µg of a HeLa whole cell lysate. This antibody detects a 27 kDa band corresponding to the epitope tag GFP.
Application

Western Blot of Rabbit anti-GFP antibody . Lane 1: 293FT cells transfected with CDK4 dominant negative (C-). Lane 2: 293FT cells poitive control (C+). Load: 25 µg per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4°C. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 27 kDa for GFP.
Application

Immuno-microscopy of Rabbit anti-GFP antibody . Monocyte derived dendritic cells and dermal macrophages were challenged and directly visualized with eGFP labeled Dengue virus to localize sequestration of virus particles in the different cells (upper). The location of the GFP was confirmed by TEM (lower magnified view) using GeneTex rabbit anti GFP Primary antibody (1:200) and a gold labeled secondary antibody.
Application

Immunofuorescence using anti-GFP antibody to label GFP-expressing glial cells (green) transplanted into lesioned rat spinal cord. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary. Image courtesy of Andrew Toft.

Application Notes	For WB: Use at a dilution of 1:5000~1:20000.:

Form	Liquid
StorageInstructions	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.02M Potassium Phosphate, 0.15M Sodium Chloride. 0.01% Sodium Azide was added as a preservative.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.