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His-tag Antibody

His-tag Antibody His-tag Antibody

Instruction Manual!

Product name: His-tag Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:His-tag antibody is supplied in PBS containing 0.02% sodium azide.
Applications:ELISA IP WB
Storage:His-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
UOM:100ug

His-tag Antibody

Catalog Number:OM280944

Product Profile

Product Name His-tag Antibody
Antibody Type Tags Antibodies
Productdescription 
							
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. His-tag is one of the more popular epitope tags for expressed recombinant proteins.
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Immunogen
His-tag antibody was raised against a synthetic peptide containing HHHHHH.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
TestedApplications 

ELISAIPWB

His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Species Reactivity

Tag

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol N/A
Alternative Names
His-tag
His
6xHis
Molecular Weight(MW) N/A
Tissue Specificity His-tag antibody will detect amino- or carboxy-terminal His-tagged recombinant proteins.

Database Links

Entrez Gene N/A
Protein Accession N/A

Application

  • Application

    Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.

  • Application

    Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 μg of His antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.

PositiveControl 1) N/A
ApplicationNotes
 His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:

Additional Information

Form Liquid
StorageInstructions  His-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Storage Buffer His-tag antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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