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V5 Tag Antibody
Instruction Manual!
| Product name: | V5 Tag Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Applications: | WB |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
V5 Tag Antibody
Catalog Number：IC224412
Product Profile
| Product Name | V5 Tag Antibody |
|---|---|
| Antibody Type | Tags Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | |
|
WB~~1:100~500:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| MolecularWeight(MW) | Da |
|---|---|
| Tissue Specificity | KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen. |
Database Links
Application
-
Application
Lanes:1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.
-
Application
Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.
| Application Notes |
WB~~1:100~500:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
