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c-Myc (phospho Thr58) antibody

c-Myc (phospho Thr58) antibody c-Myc (phospho Thr58) antibody

Instruction Manual!

Product name: c-Myc (phospho Thr58) antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHC-P WB
Storage:Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:100ug

c-Myc (phospho Thr58) antibody

Catalog Number:IC247564

Product Profile

Product Name c-Myc (phospho Thr58) antibody
Antibody Type Tags Antibodies
Productdescription 
									
Rabbit Polyclonal antibody to Myc (Phospho Thr58)Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Immunogen
Peptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHC-PWB

Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
SpeciesReactivity

HumanMouseRat

Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
MRTL
bHLHe39
c-Myc
MYC
avian myelocytomatosis viral oncogene homolog
myc-related translation/localization regulatory factor
class E basic helix-loop-helix protein 39
transcription factor p64
proto-oncogene c-Myc
myc proto-oncogene protein
v-myc a
More 
Target Myc (Phospho Thr58)
Molecular Weight(MW) 60 (note)

Database Links

Application

  • Application

    Western blot analysis of extracts from Hela cells using Myc (Phospho-Thr58) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr58) Antibody (left) or the same antibody preincubated with blocking peptide (right).

Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
StorageInstructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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