c-Myc (phospho Ser62) antibody

Instruction Manual!

Product name:	c-Myc (phospho Ser62) antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Applications:	ICC/IFIHC-PWB
Storage:	Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
UOM:	100ug

Catalog Number：IC247558

Product Name	c-Myc (phospho Ser62) antibody
Antibody Type	Tags Antibodies
Productdescription	Rabbit Polyclonal antibody to c-Myc (phospho Ser62)The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008] More
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human c-Myc. The exact sequence is proprietary.

Gene Synonyms	MYCC MYC MRTL bHLHe39 v-myc avian myelocytomatosis viral oncogene homolog c-Myc More
Target	c-Myc (phospho Ser62)
Tissue Specificity	Recognizes endogenous levels of c-Myc (pS62) protein.

Application

Western blot analysis of c-Myc (pS62) expression in A431 (A); HeLa (B); Jurkat (C) whole cell lysates.
Application

Immunofluorescent analysis of c-Myc (pS62) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Application

Immunohistochemical analysis of c-Myc (pS62) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application Notes	WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500:

Form	Liquid
StorageInstructions	Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
Storage Buffer	Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.