Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)

Instruction Manual!

Product name:	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)
Source:	Mouse
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WB, IP, IHC, IF, FC, ChIP
Storage:	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied i
UOM:	100ug

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)

Catalog Number：IC296272

Product Profile

Product Name	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-mouse IgG (H+L), F(ab')₂ Fragment was conjugated to Alexa Fluor^®594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors. More

Product Name

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								
								
									Anti-mouse IgG (H+L), F(ab')₂ Fragment was conjugated to Alexa Fluor^®594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors.
								

							
More

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Mouse
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	F(ab')₂ fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Database Links

Application

Application

Immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (IF Preferred) detected with Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (red).

Additional Information

StorageInstructions	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageInstructions

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.

Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.