Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Instruction Manual!

Product name:	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)
Source:	Mouse
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WB, IP, IHC, IF, FC, ChIP
Storage:	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent assays. Storage: Supplied in 0.1 M sodium phos
UOM:	100ug

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Catalog Number：IC296274

Product Profile

Product Name	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-Mouse IgG (H+L) F(ab')₂ Fragment was conjugated to Alexa Fluor^®555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors. More

Product Name

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								
								
									Anti-Mouse IgG (H+L) F(ab')₂ Fragment was conjugated to Alexa Fluor^®555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors.
								

							
More

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Mouse
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	F(ab')₂ fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Database Links

Application

Application

Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

Additional Information

StorageInstructions	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.