Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)

Instruction Manual!

Product name:	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)
Source:	Mouse
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WB, IP, IHC, IF, FC, ChIP
Storage:	Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details. Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not
UOM:	100ug

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)

Catalog Number：IC296275

Product Profile

Product Name	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-mouse IgG (H+L), F(ab')₂ Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in mice. More

Product Name

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								
								
									This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-mouse IgG (H+L), F(ab')₂ Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in mice.
								

							
More

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Mouse
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F_c, or human serum proteins.

Database Links

Application

Application

Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with a construct overexpressing TrCP1-GST using GST (26H1) Mouse mAb #2624. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1 and 2) or Protein G Beads (lane 3). The western blot was probed using GST (91G1) Rabbit mAb #2625.
Application

Immunoprecipitation of NIH/3T3 cell extracts using Akt (pan)(40D4) Mouse mAb #2920. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein G Beads (lane 2). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.

Additional Information

StorageInstructions	Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details. Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.