Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Instruction Manual!

Product name:	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WB, IP, IHC, IF, FC, ChIP
Storage:	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:1000 should yield acceptable results for immunofluorescent assays. Storage: Supplied in 0.1 M sodium phosphate, 0
UOM:	100ug

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Catalog Number：IC296281

Product Profile

Product Name	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-Rabbit IgG (H+L) F(ab')₂ Fragment was conjugated to Alexa Fluor^® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors. More

Product Name

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								
								
									Anti-Rabbit IgG (H+L) F(ab')₂ Fragment was conjugated to Alexa Fluor^® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors.
								

							
More

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Rabbit
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	F(ab')₂ fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Database Links

Application

Application

Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application

Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

Additional Information

StorageInstructions	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:1000 should yield acceptable results for immunofluorescent assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.