Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)

Instruction Manual!

Product name:	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)
Source:	Alligator
Purity:	>95%
Buffer Formulation:	Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Applications:	ChIP,FC,IF,IHC,IP,WB
Storage:	Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
UOM:	100ug

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)

Catalog Number：IC296284

Product Profile

Product Name	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Product Name

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Key Feature

Clonality	Monoclonal
Isotype	Cocktail
Host Species	Alligator
TestedApplications	ChIPFCIFIHCIPWB
	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:Immunofluorescent: 1:1000Flow Cytometry: 1:500:
Species Reactivity	Rabbit
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Tissue Specificity	F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.

Database Links

Application

Application

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate).

ApplicationNotes	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:Immunofluorescent: 1:1000Flow Cytometry: 1:500:

Additional Information

StorageInstructions	Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.