Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb

Instruction Manual!

Product name:	Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb
Source:	Mouse
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WBIPIHCIFFCChIP
Storage:	Storage: -20°C
UOM:	100ug

Catalog Number：ic296300

Product Name	Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb
Antibody Type	Secondary Antibodies
Productdescription	Affinity purified mouse anti-rabbit IgG (Conformation Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications.

TissueSpecificity	Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb recognizes the native rabbit IgG. It does not recognize the denatured and reduced rabbit IgG heavy (about 50 kDa) or light (about 25 kDa) chains on western blot.

Application

Western blot analysis of decreasing concentrations of total rabbit IgG, reduced and denatured in 1X SDS loading buffer with DTT, using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (Panel B), or Anti-rabbit IgG, HRP-linked Antibody (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.

StorageInstructions	Storage: -20°C
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.