Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Instruction Manual!

Product name:	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WBIPIHCIFFCChIP
Storage:	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays: Immunofluorescent: 1:500
UOM:	100ug

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Catalog Number：IC296309

Product Profile

Product Name	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-rabbit IgG (H+L) F(ab')₂ Fragment was conjugated to Alexa Fluor^® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors. More

Product Name

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								
								
									Anti-rabbit IgG (H+L) F(ab')₂ Fragment was conjugated to Alexa Fluor^® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors.
								

							
More

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Rabbit
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	F(ab')₂ fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Database Links

Application

Application

Confocal immunofluorescent analysis of mitotic HeLa cells using β-Tubulin (9F3) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red).
Application

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) compared to a nonspecific negative control antibody (red).

Additional Information

StorageInstructions	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays: Immunofluorescent: 1:500 - 1:2000 Flow Cytometry: 1:250 - 1:1000 Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageInstructions

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:

Immunofluorescent: 1:500 - 1:2000

Flow Cytometry: 1:250 - 1:1000

Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.