Anti-biotin, HRP-linked Antibody

Instruction Manual!

Product name:	Anti-biotin, HRP-linked Antibody
Source:	Biotin
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WBIPIHCIFFCChIP
Storage:	For western blots, incubate membrane and diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween(R) 20 at room temperature for one hour prior to detection. Note: Sodium azide is an inhibitor of HRP-binding and should not be included in the dilution
UOM:	100ug

Anti-biotin, HRP-linked Antibody

Catalog Number：IC296293

Product Profile

Product Name	Anti-biotin, HRP-linked Antibody
Antibody Type	Secondary Antibodies
Productdescription	Affinity purified goat anti-biotin antibody is conjugated to horseradish peroxidase. This product has been optimized to detect biotinylated protein markers.

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Biotin
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	This antibody may cross react with endogenously expressed biotinylated proteins. Careful titration may be needed. Species Reactivity: All Species Expected

Database Links

Application

Additional Information

StorageInstructions	For western blots, incubate membrane and diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween(R) 20 at room temperature for one hour prior to detection. Note: Sodium azide is an inhibitor of HRP-binding and should not be included in the dilution buffer. Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageInstructions

For western blots, incubate membrane and diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween(R) 20 at room temperature for one hour prior to detection. Note: Sodium azide is an inhibitor of HRP-binding and should not be included in the dilution buffer.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.