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EGFR Antibody

EGFR Antibody EGFR Antibody

Instruction Manual!

Product name: EGFR Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IHC-PWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

EGFR Antibody

Catalog Number:IC277707

Product Profile

Product Name EGFR Antibody
Antibody Type Primary Antibodies
Productdescription 
							
EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.1) Aifa, S., et al., Exp. Cell Res. 302(1):108-114 (2005).
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Immunogen
This EGFR antibody is generated from rabbits immunized with EGFR his fusion protein.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
TestedApplications 

IHC-PWB

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity

Human

Concentration 1mg/ml
Purification Unpurified

Target Information

Gene Symbol EGFR
Alternative Names
Epidermal growth factor receptor
Proto-oncogene c-ErbB-1
Receptor tyrosine-protein kinase erbB-1
EGFR
ERBB
ERBB1
HER1
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Molecular Weight(MW) 134 kDa

Database Links

Entrez Gene 1956
Protein Accession P00533

Application

  • Application

    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EGFR Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.

ApplicationNotes
 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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