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EGFR (phospho Tyr1197) Antibody

EGFR (phospho Tyr1197) Antibody EGFR (phospho Tyr1197) Antibody

Instruction Manual!

Product name: EGFR (phospho Tyr1197) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

EGFR (phospho Tyr1197) Antibody

Catalog Number:IC277698

Product Profile

Product Name EGFR (phospho Tyr1197) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.1) Corbalan-Garcia S, et al. (1996) Mol Cell Biolt; 16 (10): 5674-5682Kanner SB, et al. (1991) Mol Cell Biol; 11 (2): 713-720Wu TT, et al. (1998) Mol Biol Cell; 9 (7): 1661-1674O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94 (7): 3250-3255Goldstein DJ, et al. (1994) J Virol; 68 (7): 4432-4441
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Immunogen
EGFR (Phospho-Tyr1197) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y (p) -L-R) derived from Human EGFR.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol EGFR
Alternative Names
ERBB
HER1
mENA
ERBB1
PIG61
MADH2
MADR2
Receptor protein-tyrosine kinase ErbB-1
kinase EGFR
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MolecularWeight(MW)  175 kDa
Tissue Specificity This antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1197.

Database Links

Entrez Gene 1956
Protein Accession NP_005219.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1197).

  • Application

    Western blot analysis of lysed extracts from HUVEC cells untreated or treated with EGF using EGFR (Phospho-Tyr1197).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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