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RELA Antibody

RELA Antibody RELA Antibody

Instruction Manual!

Product name: RELA Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:WB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

RELA Antibody

Catalog Number:IC289756

Product Profile

Product Name RELA Antibody
Antibody Type Primary Antibodies
Productdescription 
							
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).1) Pan, W.W., et al. J. Biol. Chem. 285(45):34348-34354(2010)
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Immunogen
This RELA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human RELA.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WB

For WB starting dilution is: 1:1000:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol RELA
Alternative Names
Transcription factor p65
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
RELA
NFKB3
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Molecular Weight(MW) 60 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene 5970
Protein Accession Q04206

Application

  • Application

    Western blot analysis of lysates from HL-60, U251 MG cell line (from left to right), using RELA Antibody at 1:1000 at each lane.

Application Notes
For WB starting dilution is: 1:1000:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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