PTK2 Antibody

Instruction Manual!

Product name:	PTK2 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC289168

ProductName	PTK2 Antibody
AntibodyType	Primary Antibodies
Productdescription	PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.1) Calalb,M.B., et.al., Mol. Cell. Biol. 15 (2), 954-963 (1995) More
Immunogen	This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	PTK2
Alternative Names	Focal adhesion kinase 1 FADK 1 Focal adhesion kinase-related nonkinase FRNK Protein phosphatase 1 regulatory subunit 71 PPP1R71 Protein-tyrosine kinase 2 p125FAK pp125FAK PTK2 FAK FAK1 More
Molecular Weight(MW)	119 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Rat

Entrez Gene	5747
Protein Accession	Q05397

Application

Confocal immunofluorescent analysis of PTK2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application

Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Flow cytometric analysis of K562 cells using PTK2 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Western blot analysis of PTK2 Antibody in T47D cell line lysates (35ug/lane)
Application

Western blot analysis of PTK2 using rabbit polyclonal PTK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).

ApplicationNotes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.