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FAK (phospho Tyr397) Antibody

FAK (phospho Tyr397) Antibody FAK (phospho Tyr397) Antibody

Instruction Manual!

Product name: FAK (phospho Tyr397) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

FAK (phospho Tyr397) Antibody

Catalog Number:IC278447

Product Profile

Product Name FAK (phospho Tyr397) Antibody
Antibody Type Primary Antibodies
Productdescription
							
							
							
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Matsuya M. et. al. (1998) J. Biol. Chem. 273:1003-1014Fujita H. et. al. (1998) J. Biol. Chem. 273:26516-26521Le Romancer M. et. al. (2008) Mol. Cell 31:212-221
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Immunogen
FAK (Phospho-Tyr397) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 397 (D-D-Y (p) -A-E) derived from Human FAK.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol PTK2
Alternative Names
FAK
FADK
FAK1
FRNK
PPP1R71
p125FAK
pp125FAK
FAK
PTK2
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MolecularWeight(MW)  125 kDa
Tissue Specificity This antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397.

Database Links

Entrez Gene 5747
Protein Accession NP_001186578.1

Application

  • Application

    Western blot analysis of extract from 293 cells untreated or treated with EGF using FAK (Phospho-Tyr397) Antibody.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions   Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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