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FAK (phospho Tyr576 / Tyr577) Antibody

FAK (phospho Tyr576 / Tyr577) Antibody FAK (phospho Tyr576 / Tyr577) Antibody

Instruction Manual!

Product name: FAK (phospho Tyr576 / Tyr577) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

FAK (phospho Tyr576 / Tyr577) Antibody

Catalog Number:IC278448

Product Profile

Product Name FAK (phospho Tyr576 / Tyr577) Antibody
Antibody Type Primary Antibodies
Productdescription
							
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Parsons, J. T. et al. (2000) Oncogene 19, 5606-5613Schaller, M. D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B. S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H. C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
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Immunogen
FAK (phospho-Tyr576/Tyr577) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y (p) -Y (p) -K-A) derived from Human FAK.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IFWB

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol PTK2
Alternative Names
FAK
FADK
FAK1
FRNK
PPP1R71
p125FAK
pp125FAK
EIF4F
EIF4G
EIF4GI
FADK 1
PTK2
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MolecularWeight(MW)  125 kDa
Tissue Specificity This antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.

Database Links

Entrez Gene 5747
Protein Accession NP_005598.3

Application

  • Application

    Immunofluorescence staining of methanol-fixed HeLa cells using FAK (phospho-Tyr576/Tyr577).

  • Application

    Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577).

Application Notes
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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