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Akt (Phospho-Ser473) antibody
Instruction Manual!
| Product name: | Akt (Phospho-Ser473) antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WBIHCELISA |
| Storage: | store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-Akt (Phospho-Ser473) polyclonal antibody
Catalog Number：IC122468
Product Profile
| ProductName | Rabbit anti-Akt (Phospho-Ser473) polyclonal antibody |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | |
|
WB:1:500~1:1000
More
IHC:1:50~1:100 ELISA:1:5000 |
|
| Species Reactivity | |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | AKT1 |
|---|---|
| Gene Synonyms |
AKT
More
AKT1 kinase C-AKT kinase Akt1 PKB PKB-alpha Protein kinase B RAC RAC-alpha serine/threonine kinase RAC-PK-alpha |
Database Links
| Entrez Gene |
207 208 10000 |
|---|---|
| Unigene |
525622 631535 498292 |
| OMIM |
164730 164731 611223 |
| SwissProt ID |
P31749 P31751 Q9Y243 |
Application
-
Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is treated with the synthesized peptide.
-
Western blot
Western blot analysis of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with the synthesized peptide
| Application Notes |
WB:1:500~1:1000
More
IHC:1:50~1:100 ELISA:1:5000 |
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
