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Akt (phospho Ser473) Antibody

Akt (phospho Ser473) Antibody Akt (phospho Ser473) Antibody

Instruction Manual!

Product name: Akt (phospho Ser473) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Akt (phospho Ser473) Antibody

Catalog Number:IC270698

Product Profile

Product Name Akt (phospho Ser473) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2): 341-3. Min YH, et al. (2004) Cancer Res; 64 (15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34): 35510-7.
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Immunogen
Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol AKT1
Alternative Names
AKT
PKB
RAC
CWS6
PRKBA
PKB-ALPHA
RAC-ALPHA
NFKB3
C-AKT
PKB-alpha
RAC-PK-alpha
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Molecular Weight(MW) 60 kDa
Tissue Specificity This antibody detects endogenous level of Akt only when phosphorylated at serine 473.

Database Links

Entrez Gene 207
Protein Accession NP_001014431.1

Application

  • Application

    Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF, Serum or both using Akt (Phospho-Ser473).

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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