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Rb (phospho Ser795) Antibody

Rb (phospho Ser795) Antibody Rb (phospho Ser795) Antibody

Instruction Manual!

Product name: Rb (phospho Ser795) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFIHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Rb (phospho Ser795) Antibody

Catalog Number:IC289589


Product Profile

Product Name Rb (phospho Ser795) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.1) Fang Sun, Hanjiang Fu, Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564–1568.
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Immunogen
Rb (Phospho-Ser795) antibody was raised against a peptide sequence around phosphorylation site of serine 795 (P-S-S (p) -P-L) derived from Human Rb.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
SpeciesReactivity 

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol RB1
Alternative Names
RB
pRb
OSRC
pp110
p105-Rb
ASK1
MAPKKK5
MEKK5
P105-RB
PP105
PP110
RB-1
RB1
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Molecular Weight(MW) 110 kDa
Tissue Specificity This antibody detects endogenous level of Rb only when phosphorylated at serine 795.

Database Links

Entrez Gene 5925
Protein Accession NP_000312.2

Application

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Rb (Phospho-Ser795).

  • Application

    Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).

ApplicationNotes
 Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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