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AKT1 (phospho Thr450) Antibody
Instruction Manual!
| Product name: | AKT1 (phospho Thr450) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
AKT1 (phospho Thr450) Antibody
Catalog Number：IC270707
Product Profile
| Product Name | AKT1 (phospho Thr450) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
AKT1 (phospho-Thr450) antibody was raised against a peptide sequence around phosphorylation site of threonine 450 (T-I-T (p) -P-P) derived from Human AKT1. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names |
AKT
More
PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA CTNNB OK/SW-cl.35 PRO2286 RAC-PK-alpha Protein kinase B |
| MolecularWeight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450. |
Database Links
| Entrez Gene | 207 |
|---|---|
| Protein Accession | NP_001014431.1 |
Application
-
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450).
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450).
| ApplicationNotes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
