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Akt (phospho Thr308) Antibody

Akt (phospho Thr308) Antibody

Instruction Manual!

Product name:	Akt (phospho Thr308) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	IFIHC
Storage:	Store antibody at -20˚C for up to one year.
UOM:	100ug

Akt (phospho Thr308) Antibody

Catalog Number：IC270051

Product Profile

Product Name	Akt (phospho Thr308) Antibody
Antibody Type	Primary Antibodies
Productdescription	General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Tremblay F, et al. (2005) Diabetes; 54 (9): 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40): 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6): 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. More
Immunogen	Akt (Phospho-Thr308) antibody was raised against a peptide sequence around phosphorylation site of threonine 308 (M-K-T (p) -F-C) derived from Human Akt.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	IFIHC
	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity	MouseRat
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	AKT1
Alternative Names	AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA CFL C-AKT PKB-alpha RAC-PK-alpha More
Molecular Weight(MW)	60 kDa
Tissue Specificity	This antibody detects endogenous level of Akt only when phosphorylated at threonine 308.

Database Links

Entrez Gene	207
Protein Accession	NP_001014431.1

Application

Application

Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Akt (Phospho-Thr308).

Application Notes	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form	Liquid
StorageInstructions	Store antibody at -20˚C for up to one year.
Storage Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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