JUN Antibody

Instruction Manual!

Product name:	JUN Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC282544

Product Name	JUN Antibody
Antibody Type	Primary Antibodies
Productdescription	JUN is the putative transforming gene of avian sarcoma virus 17. JUN is a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.1) Song, J.Y., et al. J. Biol. Chem. 285(12):9067-9076(2010) More
Immunogen	This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the C-terminal region of human JUN.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	JUN
Alternative Names	Transcription factor AP-1 Activator protein 1 AP1 Proto-oncogene c-Jun V-jun avian sarcoma virus 17 oncogene homolog p39 JUN More
Molecular Weight(MW)	36 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Mouse

Entrez Gene	3725
Protein Accession	P05412

Application

JUN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application

Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Western blot analysis in U251 cell line lysates (35ug/lane).

ApplicationNotes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.